EBI3 Antilichaam

EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

10148

Q14213

NP_005746

Human

4967

Unconjugated

Primary Antibodies

Recombinant Protein Test (Figure 2) shows that anti-EBI-3 antibody (4967) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4967 antigenic peptide. Recombinant Protein Test

Immunology

EBI3 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1315 - Human Tonsil Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Mouse: (46.7%), Rat: (46.7%)

EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

EBI3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human EBI-3 has only 1 isoform (229aa, 25kD) . Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD) . 4967 can detect human EBI-3 but cannot detect mouse and rat EBI-3.

EBI3

Cat. No. 10-901 - Human Spleen Tissue Slide

Epstein-Barr virus induced 3

Homo sapiens

Pfanz et al. Immunity2002; 16:779-90.

Devergne et al. Proc. Natl. Acad. Sci. USA1997; 94:12041-6.

Niedbala et al. Eur. J. Immunol.2007; 3021-29.

Pfanz et al. J. Immunol.2004; 172:2225-31.

EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta

ELISA, WB, IHC-P, IF

14577917

Liquid