E.coli Maltose Binding Proteïne/MBP antilichaam

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

E.coli

Recombinant MBP purified from E. coli

10C12

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Maltose binding protein, malE, E.coli MBP, Escherichia coli MBP, MBP, AMB0710-10 µg, AMB0710-20 µg, AMB0710-50 µg, AMB0710-100 µg, AMB0710-250 µg, AMB0710-500 µg, AMB0710-1 mg, AMB0710-10, AMB0710-20, AMB0710-50, AMB0710-100, AMB0710-250, AMB0710-500, AMB0710-1

Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215.; ; Weiming Y et al., (2001) EMBO J 20 (3) :362-371.; ; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29) :10200-10205.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Protein Marker

NP_418458.1

P0AEX9

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MBP-tagged WLS protein (58.9kDa) ; ; Lane 2.: MBP-tagged IRF7 protein (59.3kDa) ;