EDF1 cDNA

Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas.

EDF-1, MBF1

9q34.3

Human

PATGen (puc19-derived cloning vector)

ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC ATCGAGAAGG GGCCTAGGGC GAAATGA

EDF1, EDF-1, MBF1, EDF1, ATGD0052-10 µg, ATGD0052-20 µg, ATGD0052-50 µg, ATGD0052-100 µg, ATGD0052-250 µg, ATGD0052-500 µg, ATGD0052-1 mg, ATGD0052-10, ATGD0052-20, ATGD0052-50, ATGD0052-100, ATGD0052-250, ATGD0052-500, ATGD0052-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

605107

NP_003783.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_003792.3

447bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.