EGF-eiwit (n-vet, muis)

EGF

Egf

N-cleavage

< 1.0 EU per μg of the protein as determined by the LAL method

> 95 % as determined by SDS-PAGE

1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL. 2.Human intestinal cancer organoids were cultured with RSPO1 (Cat#11083-HNAS), EGF (Cat#50482-MNCH), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 3.Human colorectum organoids were cultured with RSPO1 (Cat#11083-HNAS), EGF (Cat#50482-MNCH), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 4.Human lung cancer organoids were cultured with FGF2 (Cat#10014-HNAE), FGF4 (Cat#16043-HNAE), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 5.Human lung organoids were cultured with FGF2 (Cat#10014-HNAE), FGF4 (Cat#16043-HNAE), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 6.Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#10014-HNAE), HGF (Cat#10463-HNAS), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 7.Human liver cancer organoids were cultured with FGF2 (Cat#10014-HNAE), HGF (Cat#10463-HNAS), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), TGFB1 (Cat#10804-HNAC), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 8.Human kidney cancer organoids were cultured with FGF2 (Cat#10014-HNAE), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 9.Human kidney organoids were cultured with FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS), HGF (Cat#10463-HNAS), FGF4 (Cat#16043-HNAE) . (Routinely tested) . Data provided by D1 Medical Technology. 10.Human gastric cancer organoids were cultured with EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 11.Human stomach organoids organoids were cultured with EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 12.Human breast cancer organoids were cultured with FGF7 (Cat#10210-H07E), RSPO1 (Cat#11083-HNAS), IGF1 (Cat#10598-HNAE), EGF (Cat#50482-MNCH), NRG1 Beta 1 (Cat#11609-H01H), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 13.Human ovarian organoids were cultured with IGF1 (Cat#10598-HNAE), NRG1 Beta 1 (Cat#11609-H01H), RSPO1 (Cat#11083-HNAS), EGF (Cat#50482-MNCH), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 14.Human small intestinal organoids were cultured with IL22 (Cat#13059-HNAE), FGF10 (Cat#10573-HNAE), EGF (Cat#50482-MNCH), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology.

Asn977-Arg1029

The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.

Mouse