EGFP-antistoffen

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet) . Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

Enhanced green fluorescent protein

Recombinant EGFP (1-239aa) purified from E. coli

AT1D9

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

EGFP, ATGA0553-10 µg, ATGA0553-20 µg, ATGA0553-50 µg, ATGA0553-100 µg, ATGA0553-250 µg, ATGA0553-500 µg, ATGA0553-1 mg, ATGA0553-10, ATGA0553-20, ATGA0553-50, ATGA0553-100, ATGA0553-250, ATGA0553-500, ATGA0553-1

Phillips GJ (Oct 2001) FEMS Microbiology Letters. 204 (1) : 9-18.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Tags & Cell Markers

ABW34377

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2: EGFP Transfected 293T cell lysate;