EGFR-eiwit (C-His, mens)

EGFR

ERBB, ERBB1, ERRP, HER1, mENA, NISBD2, PIG61

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

1.Flow cytometric analysis of anti-EGFR CAR expression. 5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:125 dilution (0.8 μL stock solution in 100 μL FACS buffer) of PE-conjugated human EGFR Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated EGFR protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human EGFR Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

Met1-Ser645

The recombinant Human EGFR consists of AA Met1-Ser645 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 72 kDa.

Human