EIF3B Antibody / Eukaryotic translation initiation factor 3 subunit B

EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P55884

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids Q178-K595) was used as the immunogen for the EIF3B antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This EIF3B antibody is available for research use only.

After reconstitution, the EIF3B Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the EIF3B antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human colon tissue with EIF3B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.