EIF4A2 Polyklonale Antilichaam, Cy5,5 Vervoeging
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5 cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
ATP dependent RNA helicase eIF4A 2; BM-010; DDX2B; eIF 4A II; EIF4A; eIF4A II; EIF4A2; EIF4F; Eukaryotic initiation factor 4A II; eukaryotic translation initiation factor 4A isoform 2; Eukaryotic translation initiation factor 4A2; IF4A2_HUMAN.
1974
Q14240
Cytoplasm
• Rabbit
Rat
141-240/407
EIF4A2
• Polyclonal
• IgG
Cy5.5
KLH conjugated synthetic peptide derived from human eIF4A2
IF (IHC-P), IF (IHC-F), IF (ICC)
Purified by Protein A.
675nm/694nm
1µg/µl
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Unmodified
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
1974
Human, Mouse, Cow, Sheep, Pig, Horse, Chicken
Beschrijving
EIF4A2 Polyklonale Antilichaam, Cy5,5 Vervoeging Beschikbaar in 100 µL. Bestel eenvoudig online met snelle levering.
Specificaties
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OneTaq Hot Start 2X Master Mix with GC Buffer
NEB M0485L
500 Reactions
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