ELF3 Antilichaam / E74-achtige factor 3

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

P78545

Human

E.coli-derived human ELF3 recombinant protein (Position: Q48-L314) was used as the immunogen for the ELF3 antibody.

WB, IP, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ELF3 antibody is available for research use only.

After reconstitution, the ELF3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ELF3 antibody should be determined by the researcher.

Immunoprecipitation of ELF3 in whole cell lysate. Western blot analysis of ELF3 using anti-ELF3 antibody. Lane 1: whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ELF3 antibody in whole cell lysate, Lane 3: anti-ELF3 antibody (2ug) + whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELF3 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. ELF3 (~41 kDa predicted) was detected as bands at ~50 kDa, and ~100 kDa. The ~50 kDa form corresponds to phosphorylated/SUMO-modified ELF3, while the ~100 kDa species likely represents a dimeric or complex-associated form, consistent with published reports.