ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4)

CD252; TXGP1; OX40L; Gp34; OX40-L; Tax-Transcriptionally Activated Glycoprotein 1,34kD; Glycoprotein Gp34; OX40 ligand

P23510

Enzyme-linked immunosorbent assay for Antigen Detection.

CD & Adhesion molecule; Tumor immunity

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

3h

Serum, plasma, tissue homogenates and other biological fluids

15.6-1,000pg/mL

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The minimum detectable dose of this kit is typically less than 6.1pg/mL

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

48T, 96T, 96Tx5, 96Tx10, 96Tx100

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) . No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) and analogues was observed.

Tumor Necrosis Factor Ligand Superfamily, Member 4

Double-antibody Sandwich

Homo sapiens (Human)