Elk-1 (fosfo Ser383) Antilichaam

Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors. Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1. Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus.

2002

P19419

P19419

Human, Mouse, Rat

Elk-1 (Ser383) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser383 of human Elk-1.

Unconjugated

Primary Antibodies

Neuroscience, Phospho-Specific

Affinity Purified

Batch dependent

Phospho-Specific

Blue Ice

For long term storage -80˚ C is recommended, but shorter term storage at -20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚ C.

46

Elk-1 antibody is specific for ~46k Elk-1 phosphorylated at Ser383 using phospho and dephospho Elk-1 recombinant protein.

Immunolabeling of the Elk-1 protein band in a Western blot of recombinant Elk-1 is blocked by the phosphopeptide used as antigen but not by the corresponding non-phospho-Elk-1. Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

ELK1

ELK1, member of ETS oncogene family

Homo sapiens

Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D., and Martins-Green, M. "MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300." EMBO J. 22 (2003), 281 - 91.

Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., "Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1, " J. Neurosci. 22 (2002) 2054 - 2062.

Hill, C.S. and Treisman, R. "Transcriptional regulation by extracellular signals: mechanisms and specificity, " Cell 80 (1995), 199 - 211.

WB

12643407

Liquid