ELOC (NM 005648) Human Over-expression Lysate

Transient overexpression lysate of transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C) (TCEB1)

SIII; TCEB1

6921

NM_005648

8q21.11

HEK293T

C-Myc/DDK

Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Liquid

Druggable Genome, Transcription Factors

12.5 kDa

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

ELOC

Human

NP_005639

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.

https://www.ncbi.nlm.nih.gov/gene/6921

https://www.ncbi.nlm.nih.gov/nuccore/NM_005648

https://www.ncbi.nlm.nih.gov/nuccore/NP_005639