EndoG-antilichaam

EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

2021

Q14249

NP_004426

Human, Mouse, Rat

3035

Unconjugated

Primary Antibodies

KD validation (Figure 13, 14) : Anti-EndoG antibody (3035) specificity was further verified by EndoG specific knockdown. EndoG signal in human cancer cell lines transfected with EndoG siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3035 Localization validation (Figure 7, 8, 10, 11) : EndoG translocation was observed in different cell lines and tissues after treatment. From mitochondria to cytoplasm: figure 7 and figure 8. From cytosol to nucleus: figure 10 and figure 11. Localization validation

Apoptosis, Cancer

EndoG Antibody is affinity chromatography purified via peptide column.

Cat. No. 1211 - HepG2 Cell Lysate

1 mg/mL

EndoG Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

EndoG antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human EndoG has only one isoform (297aa, 33kD) . Mouse EndoG has one isoform (294aa, 32kD) and Rat EndoG also has one isoform (294aa, 32kD) . 3035 can detect human, mouse and rat isoform.

ENDOG

Cat. No. 1212 - 3T3 Cell Lysate

Cat. No. 1307 - Human Pancreas Tissue Lysate

Cat. No. 11-101 - Human Pancreas Tissue Slide

Endonuclease G

Homo sapiens

Li et al. Nature 2001;412 (6842) :95-9.

Parrish et al. Nature 2001;412 (6842) :90-4.

Hengartner. Nature. 2001;412 (6842) :27, 29.

Widlak et al. J Biol Chem. 2001;276 (51) :48404-9.

18504456

16151635

17292393

16689664

20574921

17955488

19459857

19808980

16728972

16046141

17050806

25865938

18699742

19793916

21377520

28122282

EndoG Antibody: Endonuclease G, mitochondrial, Endo G

ELISA, WB, IHC-P, IF

53759134

Liquid