ENOPH1-cDNA

Bifunctional enzyme that catalyzes the enolization of 2, 3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1, 2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) .

E1, MASA, MST145, mtnC

4q21.22

Human

PATGen (puc19-derived cloning vector)

ATGGTCGTGCTTTCGGTCCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACCACAACCCCGATTGCTTTCGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAAGAGTATCTGCAGACACATTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGGAAACAGGCTGAAGAGGACGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGGAATGGAGTGGATGATCTGCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAAGACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCGGCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCAGGAAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAGGCACAGAAACTGTTATTCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTTGATACCAAGATTGGACACAAAGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATTGGGTGCTCAACCAACAACATTTTGTTTCTGACAGATGTTACTCGAGAGGCCAGTGCTGCTGAGGAAGCAGATGTGCACGTAGCTGTGGTGGTGAGACCAGGCAACGCAGGATTAACAGATGATGAGAAGACTTACTACAGCCTCATCACATCCTTCAGTGAACTATACCTGCCTTCCTCAACCTAG

ENOPH1, E1, MASA, MST145, mtnC, ENOPH1, ATGD0252-10 µg, ATGD0252-20 µg, ATGD0252-50 µg, ATGD0252-100 µg, ATGD0252-250 µg, ATGD0252-500 µg, ATGD0252-1 mg, ATGD0252-10, ATGD0252-20, ATGD0252-50, ATGD0252-100, ATGD0252-250, ATGD0252-500, ATGD0252-1

Store the plasmid at -20C.

Lyophilized

Metabolism

NP_067027.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_021204.4

786bp

This shuttle vector contains the complete ORF. It is inseted Nde I to BamH I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.