ENos Antilichaam / NOS3

NOS3 (Nitric Oxide Synthase 3), also called ENOS, anitric oxide synthasethat generatesNOinblood vesselsand is involved with regulatingvascular toneby inhibitingsmooth musclecontraction and plateletaggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility tocoronary spasm. Fulton et al. (1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression ofNOS3in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

P29474

Human, Mouse, Rat

Amino acids Q1052-P1203 from the human protein were used as the immunogen for the NOS3 antibody.

WB, IHC-P, FACS, Direct ELISA

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

This NOS3 antibody is available for research use only.

Store the NOS3 antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the NOS3 antibody should be determined by the researcher.

IHC staining of FFPE human lung cancer with eNOS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.