EpCAM Antilichaam

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL

P16422

Human

Human breast cancer MCF-7 cells were used as the immunogen for the EpCAM antibody.

Ber-EP4

IHC-P, FACS

Protein G affinity

Purified

This EpCAM antibody is available for research use only.

Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Cell surface, cytoplasmic

IHC staining of FFPE human colon adenocarcinoma with EpCAM antibody (clone Ber-EP4) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.