EPHA 2/3/4 (fosfo Tyr588/596) Antilichaam

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

1969;2042;2043

P29317; P29320; P54764

NP_004422.2

Human, Mouse

EPHA 2/3/4 (phospho Tyr588/596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p) -V-R) / (R-T-Y (p) -V-D) derived from Human EPHA2/3/4.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

130 kDa

IgG

The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

EPHA2, EPHA3, EPHA4

EPH receptor A2, EPH receptor A3, EPH receptor A4

Homo sapiens

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324 (1990) .

Zhang Y., Mol. Cell. Proteomics 4:1240-1250 (2005) .

Zhuang G., J. Biol. Chem. 282:2683-2694 (2007)

ECK, MPK-5, SEK2, kinase EphA2

WB, IF

32967311

Liquid