EPS8 Antilichaam

Upon binding to EGF receptor, EPS8 enhances EGF-dependent mitogenic signals. It can bind multiple cellular targets. EPS8 is expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. It is expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. EPS8 is phosphorylated by several receptor tyrosine kinases. The protein contains 1 PH domain and 1 SH3 domain.

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

Q12929

Human

A portion of amino acids 9-39 from the human protein were used as the immunogen for the EPS8 antibody.

WB, IHC-P, FACS, IF

Antigen affinity purified

Purified

This EPS8 antibody is available for research use only.

Aliquot the EPS8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the EPS8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cytoplasmic

Western blot testing of human 1) A549 and 2) LOVO cell lysate with EPS8 antibody. Expected molecular weight: 92-97 kDa.