ERBB2-eiwit (C-AVI & zijn, mens)

ERBB2

C-ERB-2, c-ERB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, MLN19, NEU, NGL, p185 (erbB2), TKR1, VSCN2

C-AVI & His

< 1.0 EU per μg protein as determined by the LAL method.

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

1.Immobilized Anti-HER2 (Research Grade Trastuzumab biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human HER2 / ErbB2 Protein (His & AVI Tag), Biotinylated (Cat: 10004-H27H-B), the EC50 is 1-3 ng/mL (QC tested) . 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.1-0.5 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody (Routinely tested) . 3.Anti-HER2 (Research Grade Trastuzumab biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human Her2/ERBB2 Protein, His & AVI Tag (Cat. No. 10004-H27H-B) with an affinity constant of 5.302 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Immobilized Recombinant Human HER2 / ErbB2 Protein (His & AVI Tag), Biotinylated (Cat: 10004-H27H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Anti-HER2 (Research Grade Pertuzumab biosimilar), the EC50 is 3.8-11.6 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human HER2 / ErbB2 Protein (His & AVI Tag), Biotinylated (Cat: 10004-H27H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Anti-HER2 (Research Grade Pertuzu*mab Biosimilar), the EC50 is 2.2 ng/mL (Routinely tested) . 6.Immobilized Anti-HER2 (Research Grade Pertuzu*mab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human HER2 / ErbB2 Protein (His & AVI Tag), Biotinylated (Cat: 10004-H27H-B), the EC50 is 1.9 ng/mL (Routinely tested) .

Met1-Thr652

The recombinant human ERBB2 consists of 656 amino acids and predicts a molecular mass of 72.6 kDa. The molecular weight of this protein is around 88.3 kDa verified by SEC-MALS (Routinely tested) .

Human