ERBB2-eiwit (C-His, mens)

ERBB2

C-ERB-2, c-ERB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, MLN19, NEU, NGL, p185 (erbB2), TKR1, VSCN2

C-His

< 1.0 EU per μg protein as determined by the LAL method.

1.Flow cytometric analysis of anti-ERBB2 CAR expression. 5e5 of anti-ERBB2 CAR-293 cells were stained with 100 μL of 1:250 dilution of PE-conjugated human ERBB2 Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 10004-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated ERBB2 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human ERBB2 Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 10004-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.Flow cytometric analysis using PE conjugated ERBB2 protein. 5e5 of anti-ERBB2 CAR-293 cells were stained with PE-conjugated human ERBB2 protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No.10004-H86H-SP) . Non-transduced 293 cells were used as a control (Black Histogram) . The protein exhibited stable performance after multiple freeze-thaw cycles (5, 10 cycles)

Met1-Thr652

The protein has a predicted molecular mass of 72.8 kDa.

Human