ERC1 Antilichaam / ELKS/RAB6-interactief/CAST-familielid 1

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q8IUD2

Human, Mouse, Rat

E.coli-derived human ERC1 recombinant protein (Position: H721-S1116) was used as the immunogen for the ERC1 antibody.

WB, ICC/IF, IP, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ERC1 antibody is available for research use only.

After reconstitution, the ERC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ERC1 antibody should be determined by the researcher.

Cytoplasm

Immunoprecipitating ERC1 in Hela whole cell lysate. Western blot analysis of ERC1 using anti-ERC1 antibody. Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERC1 antibody in Hela whole cell lysate, Lane 3: anti-ERC1 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERC1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected band size for ERC1 is at 128 kDa.