ESPN Antilichaam / Espin

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

B1AK53

Mouse, Rat

E.coli-derived human ESPN recombinant protein (Position: H143-Y854) was used as the immunogen for the ESPN antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ESPN antibody is available for research use only.

After reconstitution, the ESPN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ESPN antibody should be determined by the researcher.

Western blot analysis of ESPN using anti-ESPN antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESPN antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Western blot probed with anti-ESPN shows a major band at ~100 kDa in rat testis and ~110 kDa in mouse testis, higher than the predicted ~92 kDa, consistent with species-specific espin isoforms and phosphorylated forms expressed during spermatogenesis.