FAN1 Polyklonale Antilichaam

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771) . Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016) . Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771) . Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771) . Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866) .

KMIN; hFAN1; MTMR15; KIAA1018; Fanconi-associated nuclease 1; FANCD2/FANCI-associated nuclease 1; Myotubularin-related protein 15; FAN1

22909

Q9Y2M0

Nucleus

Human, Mouse, Rat

400-450/1017

FAN1

Unconjugated

KLH conjugated synthetic peptide derived from human FAN1

WB

Purified by Protein A.

1µg/µl

WB (1:300-5000)

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Unmodified

Store at -20°C for 12 months.

22909