FBLIM1 cDNA

FBLIM1 is a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. FBLIM1 localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAL, FBLP-1, FBLP1

1p36.21

Human

PATGen (puc19-derived cloning vector)

ATGGCCTCAAAGCCTGAGAAGAGGGTGGCATCGTCTGTCTTTATCACCCTGGCACCCCCGCGCCGCGATGTGGCCGTGGCGGAGGAAGTGAGGCAGGCAGTTTGTGAGGCCCGGCGTGGCCGCCCCTGGGAGGCTCCTGCCCCCATGAAGACACCCGAGGCTGGCTTGGCGGGGAGGCCCAGCCCCTGGACAACCCCTGGCAGAGCTGCAGCCACAGTGCCGGCTGCACCTATGCAGCTCTTCAATGGAGGATGCCCACCCCCTCCTCCTGTCCTGGATGGTGAGGACGTGCTTCCTGACCTGGACCTCCTCCCACCCCCTCCACCGCCCCCTCCAGTGCTTCTGCCTTCTGAAGAGGAGGCTCCTGCTCCAATGGGGGCCTCACTCATTGCAGACTTAGAGCAGCTGCACCTGTCCCCGCCCCCGCCCCCACCACAGGCCCCAGCGGAGGGACCTTCAGTCCAGCCCGGTCCCCTCAGGCCCATGGAGGAAGAGCTGCCACCTCCCCCGGCAGAACCTGTTGAGAAAGGGGCATCCACAGACATCTGTGCCTTCTGCCACAAGACCGTGTCCCCCCGAGAGCTGGCTGTGGAGGCCATGAAGAGGCAGTACCATGCCCAGTGCTTCACGTGCCGCACCTGCCGCCGCCAGCTGGCTGGGCAGAGCTTCTACCAGAAGGATGGGCGACCCCTCTGCGAACCCTGCTACCAGGACACACTGGAGAGGTGCGGCAAGTGTGGCGAGGTGGTCCGGGACCACATCATCAGGGCCCTGGGCCAGGCCTTCCACCCCTCCTGCTTCACGTGTGTGACCTGCGCCCGGTGCATTGGGGATGAGAGCTTTGCCCTGGGCAGCCAGAACGAGGTGTACTGCCTGGACGACTTCTACAGGAAATTCGCCCCCGTCTGCAGCATCTGTGAAAATCCCATCATCCCTCGGGATGGGAAAGATGCCTTCAAAATCGAATGCATGGGAAGAAACTTCCATGAAAATTGCTACAGGTGTGAGGACTGCAGGATCCTCCTGTCTGTCGAGCCCACGGACCAAGGCTGCTACCCCCTGAACAACCATCTCTTCTGCAAGCCATGCCATGTGAAGCGGAGTGCTGCGGGGTGCTGCTGA

FBLIM1, CAL, FBLP-1, FBLP1, FBLIM1, ATGD0378-10 µg, ATGD0378-20 µg, ATGD0378-50 µg, ATGD0378-100 µg, ATGD0378-250 µg, ATGD0378-500 µg, ATGD0378-1 mg, ATGD0378-10, ATGD0378-20, ATGD0378-50, ATGD0378-100, ATGD0378-250, ATGD0378-500, ATGD0378-1

Store the plasmid at -20C.

Lyophilized

Cytoskeleton

607747

NP_060026.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_017556.2

1122bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.