FCGR2B eiwit (C-His, Mouse)

FCGR2B

CD32, F630109E10Rik, Fcgr2, FcgRII, Fcr-2, Fcr-3, fcRII, Fc[g]RII, Ly-17, Ly-m20, LyM-1

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

1.Loaded Mouse FcγRIIB / CD32b recombinant protein (Cat. 50030-M08H) on His1K Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 1.1 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Loaded Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar) (Mouse IgG2a) on ProA Biosensor, can bind Recombinant Mouse CD32B/Fcgr2b Protein, His Tag (Cat. No. 50030-M08H) with an affinity constant of 1.07μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3.Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag (Cat. No. 50030-M08H) on His1K Biosensor, can bind Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar) (Mouse IgG2a) with an affinity constant of 36.2nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Anti-CD3 (Research Grade OKT3 Biosimilar) immobilized on CM5 chip, can bind Recombinant Mouse CD32 / FCGR2B Protein (His Tag) (Cat: 50030-M08H) with an affinity constant of 0.672 μM as determined in an SPR assay (Biacore 8K) (QC tested) . 5.Anti-CD3 (Research Grade OKT3 Biosimilar) captured on Protein G chip, can bind Recombinant Mouse CD32 / FCGR2B Protein (His Tag) (Cat: 50030-M08H) with an affinity constant of 0.261 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 6.Loaded Anti-HER2 (Research Grade Trastuzumab Biosimilar) (Cat. No. 68046-H002) on ProG Biosensor, can bind Recombinant Mouse CD32 / FCGR2B Protein (His Tag) (Cat. No. 50030-M08H) with an affinity constant of 0.226 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met1-Arg217

The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 34 kDa due to glycosylation. The molecular weight of this protein is around 26-38 kDa verified by SEC-MALS (Routinely tested) .

Mouse