FCGRT & B2M eiwit (C-His, mens)

FCGRT & B2M

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE, ≥ 90 % as determined by SEC-HPLC, ≥ 90% as determined by SEC-MALS (Routinely tested) .

1.Loaded Human FcRn recombinant protein (Cat. CT009-H08H) on His1K Biosensor, can bind Recombinant Human IgG1-Fc Protein (103 Cys/Ser) (Cat: 10702-HNAC) with an affinity constant of 0.11 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Loaded Human FcRn recombinant protein (Cat. CT009-H08H) on His1k Biosensor, can bind Recombinant Human IgG4-Fc Protein (S108P) (Cat: 13505-HNAH) with an affinity constant of 0.08 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) captured on CM5 chip via Anti-His antibody, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 0.897 μM as determined in an SPR assay (Biacore T200) (QC tested) . 4.Captured Human FcRn recombinant protein (Cat. CT009-H08H) on Anti-His Chip can bind Anti-PD1 (Research Grade Nivolumab Biosimilar) with an affinity constant of 0.23 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT009-H08H) on His1K Biosensor, can bind Anti-HER2 (Research Grade Trastuzumab Biosimilar) with an affinity constant of 11.1nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IgG1-Fc Protein (103 Cys/Ser) (Cat: 10702-HNAH) with an affinity constant of 0.932 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 7.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IgG3-Fc / IGHG3 Protein (Cat: 13906-HNAH) with an affinity constant of 1.590 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 8.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IgG4-Fc Protein (S108P) (Cat: 13505-HNAH) with an affinity constant of 0.350 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 9.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IgG2-Fc Protein (257 Ser/Ala) (Cat: 13504-HNAH) with an affinity constant of 0.932 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 10.anti-ERBB2 (Research grade trastuz*mab biosimilar) immobilized on CM4 chip, can bind Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H) with an affinity constant of 0.536 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Met1-Ser297 & Met1-Met119

The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 45-53 kDa verified by SEC-MALS (Routinely tested) .

Human