Fibronectin Antilichaam

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb does not cross-react with MMP-2, MMP-9, or TIMP-2.

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

P02751

Human

High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody. The epitope of this mAb is located in the 29kDa N-terminal fibrin- and heparin-binding domain of fibronectin.

616

IHC-P

Protein G affinity chromatography

Purified

This Fibronectin antibody is available for research use only.

Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Connective tissue matrix

IHC staining of FFPE human prostate carcinoma with Fibronectin antibody (clone 616) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling prior to testing.