Flt-3 Ligand

Flt3 Ligand, also known as FL, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature mouse Flt3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokinelike domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail. Within the ECD, mouse Flt3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt3 Ligand, respectively. Mouse and human Flt3 Ligand show cross-species activity. Flt3 Ligand is expressed as a non-covalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of mouse Flt3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain. Both transmembrane and soluble Flt3 Ligand signal through the tyrosine kinase receptor Flt3/ Flk2.

Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04

14255

P49772

NP_038548.3

NM_013520.3

5 G3; 5 82.0 cM

Mouse

Human, Mouse, Rat

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

< 0.1 ng/µg of protein (< 1EU/µg)

> 98% by SDS-PAGE & HPLC analyses

Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 5.0 – 8.0 ng/ml.

163

Lyophilized

18.6 kDa

E. coli