FluoSiteTM Anti-CDH17 Antilichaam, PE-Labeled

CHD17 (cadherin 17), also known as LI (liver-intestine) -cadherin, is a unique member of the cadherin superfamily of proteins, as it has seven instead of the five typical cadherin domains. It is a calcium-dependent membrane-associated glycoprotein normally expressed on epithelial cells of the small intestine and colon, where it regulates intercellular adhesion. Upregulation of this protein is found in gastric cancer, colorectal and pancreatic cancer, amongst others. CHD17 has become a therapeutic target of interest, with studies using monoclonal antibodies, ADC (antibody drug conjugates) and CAR (chimeric antigen receptor) -T cells resulting in promising outcomes.

This anti-CDH17 (cadherin-17) antibody is a purified monospecific recombinant antibody, which is labeled with R-Phycoerythrin (PE) . This antibody has been tested by flow cytometry for binding to human Cadherin-17 (CDH17) on the surface of HCT116 cells.FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Q12864

This antibody recognizes human CDH17. It has not been tested in other species.

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa) .

Useful for studying the expression of human CDH17 on the cell surface by flow cytometry.

Protein A affinity chromatography from HEK293 supernatants.

≥ 90%

Aqueous buffer solution.

+4°C (Gel Packs)

At least 6 months at +4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between +2°C and +8°C.

8 mM Phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.