FluoSiteTM Anti-PSMA Antilichaam, PE-Labeled

PSMA (prostate-specific membrane antigen), also known as Folate hydrolase 1 or FOLH1, is an enzyme with folate hydrolase and peptidase activity. It is highly expressed in prostate cancer cells and is used as a diagnostic and prognostic indicator of prostate cancer. PSMA is used in the clinic as a target for PET (positron emission tomography) imaging in the detection and progression of prostate cancer, but radioactive analogs are used for the treatment of prostate cancer. It is also expressed in other tumor types and in a few normal tissues. PSMA is under extensive investigation as target for CAR-T cells and bi-specific antibodies-based cancer therapies.

This human anti-PSMA antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE) . This antibody has been tested by flow cytometry assay for binding to human PSMA on the surface of the PSMA CHO Recombinant Cell Line (BPS Bioscience #79641) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Q04609

This antibody recognizes human PSMA. It has not been tested in other species.

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa) .

Useful for studying expression of PSMA on the cell surface by flow cytometry. For flow cytometry staining, the suggested use for this reagent is 5 µl (1 µg antibody) per million cells in 100 µl of staining volume.

Protein A affinity chromatography from HEK293 supernatants.

≥ 90%

Aqueous buffer solution

+4°C (Gel Packs)

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

8 mM Phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.