Fosfo-CHEK1 (Ser286) Polyklonale Antilichaam, APC-vervoeging

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].

Chk1 (phospho S286) ; p-Chk1 (phospho S286) ; Chk1 (Phospho-Ser286) ; CHEK1 (phospho Ser286) ; CHEK1 (phospho Ser286) ; p-CHEK1 (Ser286) ; Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe) ; CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe) ; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) ; C85740; CHK1_HUMAN.

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O14757

Ser286

Cytoplasm, Nucleus

Mouse

Phospho-CHEK1 (Ser286)

APC

KLH conjugated Synthesised phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser286.

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purified by Protein A.

650nm/660nm

1µg/µl

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Phosphorylation

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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Human, Dog, Pig, Horse, Chicken