Fosfo-CrkL (Tyr207) Antilichaam / Crk als eiwit

Western blot: 1:500-1:2000

P46109

Human

A synthesized peptide derived from human Phospho-CrkL (Y207) was used as the immunogen for the Phospho-CrkL (Tyr207) antibody.

32C08

WB

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Phospho-CrkL (Tyr207) antibody is available for research use only.

Store the Phospho-CrkL (Tyr207) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Phospho-CrkL (Tyr207) antibody should be determined by the researcher.

Human K562 cell lysate tested with the Phospho-CrkL (Tyr207) antibody at 1:1000 dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.