Fosfo-IRF3 (Ser386) Polyklonale Antilichaam

Key transcriptional regulator of type I interferon (IFN) -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Interferon regulatory factor 3; IRF-3; IRF3

3661

Q14653

Ser386

Cytoplasm, Nucleus

Human, Mouse, Rat

Phospho-IRF3 (Ser386)

Unconjugated

KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser386

WB, IHC-P, IHC-F, IF, Flow-Cyt

Purified by Protein A.

1µg/µl

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), Flow-Cyt (1ug/Test)

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Phosphorylation

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

3661