Fosfo-Nucleophosmin (Ser125) Antilichaam / NPM1

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

P06748

Human, Mouse, Rat

A synthesized peptide derived from human Phospho-Nucleophosmin (S125) was used as the immunogen for the Phospho-Nucleophosmin (Ser125) antibody.

32N71

WB, IHC

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Phospho-Nucleophosmin (Ser125) antibody is available for research use only.

Store the Phospho-Nucleophosmin (Ser125) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Phospho-Nucleophosmin (Ser125) antibody should be determined by the researcher.

Western blot testing of human samples using the Phospho-Nucleophosmin (Ser125) antibody at 1:3000 dilution for 1 hour at room temperature. A predominant band is observed at ~35 kDa, consistent with phosphorylated NPM1 migrating slightly above its predicted 33 kDa. An additional ~26 kDa species is detected, consistent with a Ser125-containing proteolytic fragment of NPM1 that appears in some cell lysates.