Fosfo-RASA1 (Tyr460) Polyklonale Antilichaam, Cy3 Vervoeging

The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1,2) . p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3) . Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, hydrolysis of their bound GTP to GDP, is impaired as a result of specific mutations (3) . Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4,5) . The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6,7), and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, including Rho, Rab 3A and Rap 1, have also been described (8,9) .

GAP phospho Y460; p-GAP phospho Y460; Ras GAP; CM AVM; CMAVM; DKFZp434N071; GAP; GTPase activating protein; GTPase-activating protein; OTTHUMP00000222390; OTTHUMP00000222391; OTTHUMP00000222392; OTTHUMP00000222393; p120GAP; p120RASGAP; PKWS; Ras GTPase-activating protein 1; RAS p21 protein activator GTPase activating protein 1; Ras p21 protein activator; RASA; RASA1; RASA1_HUMAN; RasGAP; Triphosphatase activating protein.

5921

Tyr460

Cytoplasm

Mouse

Phospho-RASA1 (Tyr460)

Cy3

KLH conjugated synthetic phosphopeptide derived from human GAP around the phosphorylation site of Tyr460

IF (IHC-P), IF (IHC-F), IF (ICC)

Purified by Protein A.

512,550nm/570,615nm

1µg/µl

IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Phosphorylation

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

5921

Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken