Fosfo-Src (Tyr529) Antilichaam

Western blot: 1:500-1:2000, Immunocytochemistry/Immunofluorescence: 1:50-1:200

P12931

Human, Mouse, Rat

A synthesized peptide derived from human Phospho-Src (Y529) was used as the immunogen for the Phospho-Src (Tyr529) antibody.

32S01

WB, ICC, IF

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Phospho-Src (Tyr529) antibody is available for research use only.

Store the Phospho-Src (Tyr529) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Phospho-Src (Tyr529) antibody should be determined by the researcher.

All lanes use the antibody at 1:3K dilution for 1 hour at room temperature. Expected size for c-Src (phospho-Tyr529) ~60 kDa. Observed (possible) doublet at ~55 kDa and ~60 kDa likely reflects full-length and slightly modified/truncated phosphorylated forms of c-Src, consistent with literature indicating Src family kinases migrate in the 55-62 kDa range.