Fosfo-Syk-antilichaam (pY525/526)

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Western blot: 1:100-1:250, Immunohistochemistry (FFPE) : 1:50-1:100

P43405

Human

This phospho-Syk antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to the amino acid residues surrounding pY525/526 of human Syk.

WB, IHC-P

Antigen affinity

Antigen affinity purified

This phospho-Syk antibody is available for research use only.

Aliquot the phospho-Syk antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

Titration of the phospho-Syk antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western blot testing of lysate from human Raji cells that was untreated (-) and treated with 200 nm TPA for 30 min (+) .