Fosforyl Histone H3 Antilichaam / pS10

Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%) . Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002) . For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P68431

Human

Amino acids ARK-pS-TGGKAPRKQLc of Phosphohistone H3 (phospho S10) were used as the immunogen for the Phosphorylated Histone H3 antibody.

RHH3/9924

IHC-P, WB

Purified

This Phosphorylated Histone H3 antibody is available for research use only.

Aliquot the Phosphorylated Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Phosphorylated Histone H3 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human tonsil tissue with Phosphorylated Histone H3 antibody (clone rHH3/9924) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.