Fosfotyrosine Antilichaam [PY20] (HRP)

Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.

Phosphotyrosine conjugated to carrier proteins

[PY20]

HRP

Primary Antibodies

Obesity, Signal Transduction, Neuroscience, Cancer

Batch dependent

Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added

None

Blue Ice

Store vial at 2-8˚ C

IgG2b

Phosphotyrosine

Phosphotyrosine Antibody [PY20] for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

PTyr20

ELISA

Liquid