FOXM1 Antilichaam / Vorkhoofd doos eiwit M1

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate

Q08050

Human, Mouse, Rat

E.coli-derived human FOXM1 recombinant protein (Position: Q457-P760) was used as the immunogen for the FOXM1 antibody.

WB, IHC, ELISA, IP

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This FOXM1 antibody is available for research use only.

After reconstitution, the FOXM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the FOXM1 antibody should be determined by the researcher.

Western blot analysis of FOXM1 using anti-FOXM1 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human DLD1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Major bands between ~85-105 kDa correspond to FOXM1 isoforms and phosphorylation states. The multiple closely spaced bands represent differentially phosphorylated forms of FOXM1, consistent with its activation during the G2/M phase of the cell cycle.