FOXO1 Antilichaam / Boorddoos eiwit O1

FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t (2;13) and t (1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxyterminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

Q12778

Human

A portion of amino acids 450-650 from human FOXO1 protein was used as the immunogen for the FOXO1 antibody.

FOXO1/9792

IHC-P, WB

Protein G affinity

Purified

This FOXO1 antibody is available for research use only.

Aliquot the FOXO1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the FOXO1 antibody should be determined by the researcher.

Cytoplasm, Nucleus

Western blot testing of human brain tissue lysate with FOXO1 antibody (clone FOXO1/9792) . Predicted molecular weight ~70 kDa.