FRP-1, oplosbaar

Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Recombinant human sFRP-1 is a glycosylated 283 amino acid protein containing a cysteine rich Frizzled homologous domain.

Soluble Frizzled Related Protein-1, Secreted Apoptosis Related protein-2, SARP-2

6422

Q8N474

NP_003003.3

NM_003012.4

8p11.21

Human

Mouse

SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK

< 0.1 ng/µg of protein (< 1EU/µg)

> 97% by SDS-PAGE & HPLC analyses

Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3–0.5 μg/ml.

283

Lyophilized

32.5 kDa

HeLa cells