G-361 Lysaat

G-361 cells are established from a malignant melanoma of a 31 year-old male. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR) -PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Human

WB

2 mg/mL (determined by BCA assay)

G361, ATGL0012-10 µg, ATGL0012-20 µg, ATGL0012-50 µg, ATGL0012-100 µg, ATGL0012-250 µg, ATGL0012-500 µg, ATGL0012-1 mg, ATGL0012-10, ATGL0012-20, ATGL0012-50, ATGL0012-100, ATGL0012-250, ATGL0012-500, ATGL0012-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : G-361 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody