G-CSF/CSF3-eiwit (hFc-tag)

Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh) G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

C17orf33 Protein; CSF3OS Protein; GCSF Protein; MGC45931 Protein

NP_757373.1

Human

Human

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE

1.Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 0.2-0.8 ng/mL. 2.Immobilized Recombinant Human G-CSFR / CD114 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human G-CSF / CSF3 Protein (Fc Tag), the EC50 is 5-15 ng/mL.

412

Lyophilized

PBS, pH 7.4

See Datasheet

48 kDa (glycosylated monomer)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells