GADD153 Antilichaam / CHOP / DDIT3

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Western blot: 1:1000-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

P35638

Human

A portion of amino acids 120-149 from the human protein were used as the immunogen for the GADD153 antibody.

WB, FACS, IF

Antigen affinity purified

Purified

This GADD153 antibody is available for research use only.

Aliquot the GADD153 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the GADD153 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Nuclear, cytoplasmic

Western blot testing of human PC-3 cell lysate with GADD153 antibody. Expected molecular weight: 19-29 kDa.