GALNT2 Antilichaam/polypeptide N-acetylgalactosaminyltransferase 2

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

Q10471

Human, Rat

E.coli-derived human GALNT2 recombinant protein (Position: H442-Q571) was used as the immunogen for the GALNT2 antibody.

WB, IP, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This GALNT2 antibody is available for research use only.

After reconstitution, the GALNT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the GALNT2 antibody should be determined by the researcher.

Immunoprecipitating (IP) GALNT2 in Hela whole cell lysate. Western blot analysis of GALNT2 using anti-GALNT2 antibody; Lane 1: Hela whole cell lysates (30ug) ; Lane 2: Rabbit control IgG instead of anti-GALNT2 antibody in Hela whole cell lysate; Lane 3: anti-GALNT2 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GALNT2 antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. Western blot analysis of GALNT2 shows a ~65 kDa band that appears as a tight doublet in some samples, consistent with differential N-glycosylation and maturation states of this Golgi glycosyltransferase.