GbetaL Antilichaam / LST8 / MLST8

Target of rapamycin complex subunit LST8, also known as mammalian lethal with SEC13 protein 8 (mLST8) or TORC subunit LST8 or G protein beta subunit-like (GbetaL or Gable), is a protein that in humans is encoded by the MLST8 (MTOR associated protein, LST8 homolog) gene. mTOR is a serine/threonine kinase that acts in two distinct complexes, mTORC1 and mTORC2, and is dysregulated in many diseases including cancer. mLST8 is a shared component of both mTORC1 and mTORC2. Mammalian lethal with SEC13 protein 8 (mLST8), is an indispensable protein subunit of mammalian target of rapamycin (mTOR) signaling pathway that interacts with the kinase domain of mTOR protein, thereby stabilizing its active site.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q9BVC4

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids D11-G326) was used as the immunogen for the GbetaL antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This GbetaL antibody is available for research use only.

After reconstitution, the GbetaL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the GbetaL antibody should be determined by the researcher.

Cytoplasm, Nucleus, Cell Junctions

Immunofluorescent staining of FFPE human lung cancer tissue with GbetaL antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.