GBX2 cDNA

GBX2 (gastrulation brain homeobox 2) is a homeobox gene involved in the normal development of rhombomeres 1-3 which is the mid/hindbrain region. This gene is a dosage dependent transcription factor involved in the regulation of proper expression of other genes. GBX2 expression occurs during gastrulation and continues to be expressed in the later stages of embryogenesis.

2q37.2

Human

PATGen (puc19-derived cloning vector)

ATGAGCGCAGCGTTCCCGCCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGCACCGCCTTCAGCATAGACTCGCTGATCGGCAGCCCGCCGCAGCCCAGCCCCGGCCATTTCGTCTACACCGGCTACCCCATGTTCATGCCCTACCGGCCGGTAGTGCTGCCGCCGCCGCCGCCGCCGCCGCCCGCGCTGCCCCAGGCCGCGCTGCAGCCAGCGCTGCCGCCCGCACACCCTCACCACCAGATCCCCAGCCTGCCCACAGGCTTCTGCTCCAGCCTGGCGCAGGGCATGGCGCTCACCTCTACGCTCATGGCCACGCTCCCCGGCGGCTTCTCCGCGTCGCCCCAGCACCAGGAGGCGGCAGCGGCCCGCAAGTTCGCGCCGCAGCCGCTGCCCGGCGGCGGTAACTTCGACAAGGCGGAGGCGCTGCAGGCTGACGCGGAGGACGGCAAAGGCTTCCTGGCCAAAGAGGGCTCGCTGCTCGCCTTCTCCGCGGCCGAGACGGTGCAGGCTTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAAAGGTGGAAGACGACCCGAAGGGCAAGGAGGAGAGCTTCTCGCTGGAGAGCGATGTGGACTACAGCTCGGATGACAATCTGACTGGCCAGGCAGCTCACAAGGAGGAAGACCCGGGCCACGCGCTGGAGGAGACCCCGCCGAGCAGCGGCGCCGCGGGCAGCACCACGTCTACGGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCGAGCAGCTGCTGGAGCTAGAGAAGGAGTTCCACTGCAAAAAGTACCTCTCCTTGACCGAGCGCTCGCAGATCGCCCACGCCCTCAAACTCAGCGAGGTGCAGGTGAAAATCTGGTTCCAGAACCGACGGGCCAAGTGGAAACGGGTGAAGGCAGGCAATGCCAATTCCAAGACAGGGGAGCCCTCCCGGAACCCTAAGATCGTCGTCCCCATCCCTGTCCACGTCAGCAGGTTCGCTATCAGAAGTCAGCATCAGCAGCTAGAACAGGCCCGGCCCTGA

GBX2, GBX2, ATGD0322-10 µg, ATGD0322-20 µg, ATGD0322-50 µg, ATGD0322-100 µg, ATGD0322-250 µg, ATGD0322-500 µg, ATGD0322-1 mg, ATGD0322-10, ATGD0322-20, ATGD0322-50, ATGD0322-100, ATGD0322-250, ATGD0322-500, ATGD0322-1

Store the plasmid at -20C.

Lyophilized

Epigenetics and Nuclear Signaling

601135

NP_001476.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001485.3

1047bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.