GDF 2

GDF-2 belongs to the TGF-ß cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-2 is expressed mainly in nonparenchymal cells of the liver, but is also found in other various cells and tissues. GDF-2 can signal through the ALK1 receptor and has been implicated in a number of physiologic events. These include regulation of the hepatic reticuloendothelial system, glucose homeostasis, and iron homeostasis, as well as the inhibition of angiogenesis. Recombinant human GDF-2 is a 24.1 kDa disulfide linked homodimeric protein consisting of two 110 amino acid polypeptide chains.

GDF2; BMP9; BMP-9

2658

Q9UK05

NP_057288

NM_016204

10q11.22

Human

Mouse

SAGAGSHCQK TSLRVNFEDI GWDSWIIAPK EYEAYECKGG CFFPLADDVT PTKHAIVQTL VHLKFPTKVG KACCVPTKLS PISVLYKDDM GVPTLKYHYE GMSVAECGCR

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

< 0.1 ng/µg of protein (< 1EU/µg)

> 95% by SDS-PAGE & HPLC analyses

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.

110

Lyophilized

Water

24.1 kDa

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

CHO cells