GDF-3

GDF-3 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Unlike most TGF-β family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes. Recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.

GDF3; KFS3; MCOP7; MCOPCB6

9573

Q9NR23

NP_065685.1

NM_020634

12p13.1

Human

Mouse

AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

< 0.1 ng/µg of protein (< 1EU/µg)

> 98% by SDS-PAGE & HPLC analyses

Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 100-150 ng/ml.

114

Lyophilized

26 kDa

E. coli